LOCUS       sequence-63953-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Piatkevich KD, Malashkevich VN, Morozova KS, Nemkovich NA, Almo SC, 
            Verkhusha VV
  TITLE     Extended Stokes shift in fluorescent proteins: chromophore-protein 
            interactions in a near-infrared TagRFP675 variant.
  JOURNAL   Sci Rep. 2013;3:1847. doi: 10.1038/srep01847.
  PUBMED    23677204
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1847. doi"; pages: " 10.1038/srep01847"
COMMENT     Sequence Label: pBAD/HisD-TagRFP675
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             112..813
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMMYPADGGLEGRNYMALK
                     LVGGGHLICSLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(859..876)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt tttgggctaa
       61 caggaggaat taaccatggg gagccaccat caccatcacc atggcagatc tatgagcgag
      121 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
      181 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac ccaaagaatc
      241 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
      301 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctg gaagcagtcc
      361 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      421 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
      481 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
      541 accgagatga tgtaccccgc tgacggcggc ctggaaggca gaaactacat ggccctgaag
      601 ctcgtgggcg ggggccacct gatctgcagc ttgaagacca catacagatc caagaaaccc
      661 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc gcagactgga aagaatcaag
      721 gaggccgaca aagagaccta tgtcgagcag cacgaggtgg ctgtggccag atactgcgac
      781 ctccctagca aactggggca caagcttaat taagaattcg aagcttggct gttttggtgg
      841 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
      901 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
      961 agtgaaacgc cgtagcgccg atggtagtgt ggggtct
//
