LOCUS       sequence-63954-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Piatkevich KD, Malashkevich VN, Morozova KS, Nemkovich NA, Almo SC, 
            Verkhusha VV
  TITLE     Extended Stokes shift in fluorescent proteins: chromophore-protein 
            interactions in a near-infrared TagRFP675 variant.
  JOURNAL   Sci Rep. 2013;3:1847. doi: 10.1038/srep01847.
  PUBMED    23677204
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:1847. doi"; pages: " 10.1038/srep01847"
COMMENT     Sequence Label: pTagRFP675-N1
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    32..112
                     /label=MCS
                     /note="multiple cloning site"
     CDS             120..821
                     /codon_start=1
                     /product="far-red monomeric fluorescent protein with an 
                     emission peak at 657 nm (Morozova et al., 2010)"
                     /label=TagRFP657
                     /note="derived from mKate"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKV
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMMYPADGGLEGRNYMALK
                     LVGGGHLICSLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgagc
       61 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca
      121 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
      181 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagaccc
      241 aaagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
      301 gcttcatgta cggcagcaag accttcatca accacaccca gggcatcccc gacttctgga
      361 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
      421 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
      481 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
      541 aggccaacac cgagatgatg taccccgctg acggcggcct ggaaggcaga aactacatgg
      601 ccctgaagct cgtgggcggg ggccacctga tctgcagctt gaagaccaca tacagatcca
      661 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccgc agactggaaa
      721 gaatcaagga ggccgacaaa gagacctatg tcgagcagca cgaggtggct gtggccagat
      781 actgcgacct ccctagcaaa ctggggcaca agcttaatta aagcggccgc gactctagat
      841 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      901 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      961 ttataatggt tacaaataaa gcaatagcat
//
