LOCUS       sequence-64070-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength GCaMP2-based genetically encoded calcium 
            indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreanu
            L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, 
            Svoboda K, Looger LL.
  TITLE     Imaging neural activity in worms, flies and mice with improved GCaMP
            calcium indicators.
  JOURNAL   Nat Methods. 2009 Dec;6(12):875-81.
  PUBMED    19898485
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-12"; volume: "6(12)"; pages: "875-81"
COMMENT     Sequence Label: G-CaMP3
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(140..583)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     699..718
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
       61 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      121 gatctagagt cgcggccgct cacttcgctg tcatcatttg tacaaactct tcgtagttta
      181 cctgaccatc cccatcgatg tctgcttccc tgatcatttc atcaacctct tcatctgtta
      241 acttctctcc aaggtttgtc atcacgtggc gaagctctgc tgcactgatg tagccattgc
      301 catccttatc aaacacacgg aacgcttctc taatttcttc ttcactgtct gtgtctttca
      361 tttttcttgc catcattgtc aggaactcag ggaagtcgat tgtgccgtca ccgtcggcat
      421 ctacttcatt gatcatgtcc tgcagctctg cttctgtggg gttctgcccc agagaccgca
      481 tcaccgtccc cagctccttg gttgttattg tcccatcccc gtccttgtca aatagggaga
      541 aagcctcttt aaattctgcg atctgctctt cagtcagttg gtcacgcgtg ttgtactcca
      601 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
      661 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
      721 tgaagaagat ggtgcgctcc tggatgtagc cttcgggcat ggcggacttg aagaagtcgt
      781 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
      841 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
      901 tgccgtag
//
