LOCUS       sequence-64084-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST EIF2C2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..66)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    119..143
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      811..820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttaaacgggc cctctagact cgagcgtaga atcgagaccg aggagagggt tagggatagg
       61 cttaccttcg aaccgcgggc cctctagact tgagcggccg ccactgtgct ggatatcaac
      121 cactttgtac aagaaagctg ggtctagata tctcgagtgc ggccgctcaa gcaaagtaca
      181 tggtgcgcag agtgtcttgg tgaacctgga ccgccttggc cagtgcttgg tggtctcgcc
      241 cgttactctg cccagaggta tggcttcctt cagcactgtc atgttcctta tccaccaggt
      301 ggtacctggc ccggaaggcc accaggtgag cgtagtatgc tggcgctggg atggacacgg
      361 agcgtgtgca gcgcacgtag gtgtgacaca gctggtaggt taggatctgc agctcatcag
      421 aggagaaacg attgtcgtcc cagaggacgt gatagtgcga aggcctgctt gtcccctgga
      481 tgccagcgtg actacacagg tagaagtcga actcggtggg gtgggtgatt ttcgtgtcca
      541 cagtcgtgcc tgctggaatg tttccacttt tcccaacccg ctcgttcttg tcagtgcaga
      601 agagccgggt gtggtgcctc ttctgcacca cgatgaaggt gatcccgggc tggtagtctt
      661 tttctagctt gatacaggcc tcacggatgg ccagcaactc gtggtggaga acctgctgga
      721 actggccttc agagacaccg tcgcggtaga agatgatgcg ggtgggcttg aagcgcgtgg
      781 acttgtagaa ctggatgagg agctcgcgga ccatggcggc caggtcttgt atgatctcct
      841 gccggtgctg ctgcacgcgc acggtggcgc a
//
