LOCUS       sequence-64099-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Barria A, Malinow R
  TITLE     Subunit-specific NMDA receptor trafficking to synapses.
  JOURNAL   Neuron. 2002 Jul 18;35(2):345-53.
  PUBMED    12160751
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2002-07-18"; volume: "35(2)"; pages: "345-53"
COMMENT     Sequence Label: pCI-EGFP-NR2a wt
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(285..287,288..800)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(327..348)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(588..607)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
       61 taggctagac gcgtagatct cacgtgagca tgcgggaacc cgctaaacct ggtggctgct
      121 gaggcggccg agatgctcgt gcgcgcagca cgccccattg catcctccac cttctccggc
      181 tacagggacc ctaagtggcg accatgggca gattgggcta ctggaccttg ctggtattgc
      241 cggcccttct ggtctggcgc gatccggcgc agaacgcggc tagggtgagc aagggcgagg
      301 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      361 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      421 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      481 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      541 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      601 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      661 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      721 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      781 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      841 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
      901 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cct
//
