LOCUS       sequence-64170-e        1980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Mank M, Santos AF, Direnberger S, Mrsic-Flogel TD, Hofer SB, Stein 
            V, Hendel T, Reiff DF, Levelt C, Borst A, Bonhoeffer T, Hubener M, 
            Griesbeck O
  TITLE     A genetically encoded calcium indicator for chronic in vivo 
            two-photon imaging.
  JOURNAL   Nat Methods. 2008 Sep;5(9):805-11. doi: 10.1038/nmeth.1243.
  PUBMED    19160515
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-09"; volume: "5"; issue: 
            "9"; pages: "805-11"
COMMENT     Sequence Label:  TN-XXL pRSETB
FEATURES             Location/Qualifiers
     source          1..1980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..69
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(73..81,82..96)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(115..117,118..120,121..633)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             1234..1968
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRG
                     EGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYIMADKQKNGIKANFKIRHNIE"
ORIGIN
        1 catatgcggg gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag
       61 caaatgggtc gggatctgta cgacgatgac gataaggatc ccgcggccgc caccatggtg
      121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat cttcttcaag
      421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      601 aaggcccact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      781 gagttcgtga ccgccgcccg catgctgagc gaggaagagc tggccaactg cttccgcatc
      841 ttcgacaagg acgctaacgg gttcatcgac atcgaggagc tgggtgagat tctcagggcc
      901 actggggagc acgtcaccga ggaggacata gaagacctca tgaaggattc agacaagaac
      961 aatgacggcc gcattgactt cgatgagttc ctgaagatga tggagggcgt gcagggtacc
     1021 agcgaggaag agctggccaa ctgcttccgc atcttcgaca aggacgctaa cgggttcatc
     1081 gacatcgagg agctgggtga gattctcagg gccactgggg agcacgtcac cgaggaggac
     1141 atagaagacc tcatgaagga ttcagacaag aacaatgacg gccgcattga cttcgatgag
     1201 ttcctgaaga tgatggaggg cgtgcaggag ctcatgggcg gcgtgcagct cgccgaccac
     1261 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1321 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1381 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1441 ggcggcagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1501 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc gcggcgaggg cgagggcgat
     1561 gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1621 tggcccaccc tcgtgaccac cctgggctac ggcctgatgt gcttcgcccg ctaccccgac
     1681 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1741 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1801 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1861 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1921 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga ctaagaattc
//
