LOCUS       sequence-64199-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Cohen Lab VIP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJMK019
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(16..37)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(250..306)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      318..327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(334..348,349..357))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(361..393)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(397..414)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
       61 tttcgaaagt ttggactgca cgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      121 gccgatgggg gtgttctgct ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt
      181 gtggcggatc ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcct tgatatagac
      241 gttctcgagt gagctcagcc gacctatagc tctgactgcg tgacctgtct tattccactt
      301 acgacgtgat gagtcgacca tggtggcgag atccttatcg tcatcgtcgt acagatcccg
      361 acccatttgc tgtccaccag tcatgctagc cataccatga tgatgatgat gatgagaacc
      421 tgatgcgcca ctaccggttc ggtcggcggc actgacatcg gcaccggcac tgggttctgg
      481 tgcctcggtg tcgcccagaa tagcccggga tctcaacagg ataaagccaa agccgacctt
      541 ggcagtcacg tccaacacca taaacagcag agtttcgatg cccaggccca ccacgccagc
      601 gccctcagtg cctatgatcc acaggatagg gtaagcggtc cacagcacca agaccagagc
      661 tgtcagggtg ttaaaggtag atgccacctc ggggccccgc tcctttgcag cagatcgcag
      721 ggatgtagcc agaaaataga gcaccactat catgcaaatt gt
//
