LOCUS       sequence-64203-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     PRT100A
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PBK-PRT100AH
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(275..292)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(275..291)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(284..306)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(500..519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     619..641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(679..697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        765..869
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttggagag gactcgagga attcggtacc ggatcctcta gagtccgcaa aaatcaccag
       61 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
      121 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
      181 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
      241 aaaatccagt gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
      301 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      361 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
      421 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
      481 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      601 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      661 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      721 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      961 taaaagatgc tgaaa
//
