LOCUS       sequence-64264-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, 
            Baker D
  TITLE     Principles for designing ideal protein structures.
  JOURNAL   Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
  PUBMED    23135467
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-11-8- 8"; volume: "491"; issue: "7423"; pages:
            "222-7"
COMMENT     Sequence Label: Di-IV_5_PL2x2_6
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             465..482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(535..553)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      549..596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(720..739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgg gcaaagtgct gctggtcatc agtaccgaca
      181 ccaacatcat ttcgtcagtc caagaacgtg ctaaacacaa ctatccgggc cgtgaaattc
      241 gtaccgcgac cagcagccag gatatccgcg acatcatcaa aagtatgaaa gataacggca
      301 aaccgctggt tgtgtttgtt aatggtgcgt cccagaacga cgtcaacgaa tttcaaaacg
      361 aagccaaaaa agaaggcgtc agctatgatg tgctgaaatc caccgacccg gaagaactga
      421 cgcaacgtgt ccgtgaattt ctgaaaaccg ctggctccct cgagcaccac caccaccacc
      481 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      541 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      601 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      661 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      721 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      781 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaac
      841 tgatagtg
//
