LOCUS       sequence-64265-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, 
            Baker D
  TITLE     Principles for designing ideal protein structures.
  JOURNAL   Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
  PUBMED    23135467
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-11-8- 8"; volume: "491"; issue: "7423"; pages:
            "222-7"
COMMENT     Sequence Label: Di-V_7_R3x1_7
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             459..476
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(529..547)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      543..590
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(714..733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgg gtagcaaaat tattgttatt attagtagcg
      181 atgacaccac cctggaagaa ctggcacgta aaatcaaaga tgaaggtctg gaagtgtaca
      241 tcctgctgaa agataaagac gaaaaacgtc tggaagaaaa aatccagaaa ctgaaaagcc
      301 aaggctttga agtgcgcaaa gttaaagatg acgatgacat cgataaatgg atcgataaaa
      361 tcaaaaaaga acgtccgcag ctggaagtcc gcaaagtcac ggacgaagac caagcgaaac
      421 aaatcctgga agacctgaaa aaaaaaggct ctctcgagca ccaccaccac caccactgag
      481 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      541 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      601 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      841 ttagggtgat ggttcacgta gtgggccatc gcc
//
