LOCUS       sequence-64266-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, 
            Baker D
  TITLE     Principles for designing ideal protein structures.
  JOURNAL   Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
  PUBMED    23135467
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-11-8- 8"; volume: "491"; issue: "7423"; pages:
            "222-7"
COMMENT     Sequence Label: Di-III_14_IF3_14
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             378..395
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(448..466)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      462..509
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(633..652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     843..864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgg gtctgacgcg caccatcacg agccaaaaca
      181 aagaagaact gctggaaatc gcactgaaat tcatctcaca aggtctggat ctggaagtgg
      241 aatttgatag caccgatgac aaagaaattg aagaattcga acgtgatatg gaagacctgg
      301 cgaagaaaac cggtgtccaa atccaaaaac aatggcaagg caacaaactg cgtatccgtc
      361 tgaaaggttc gctcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      421 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      841 tagtggactc ttgttccaaa ctggaacaac actca
//
