LOCUS       sequence-64267-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, 
            Baker D
  TITLE     Principles for designing ideal protein structures.
  JOURNAL   Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
  PUBMED    23135467
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-11-8- 8"; volume: "491"; issue: "7423"; pages:
            "222-7"
COMMENT     Sequence Label: Di-I_5_FR28
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    78..102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             117..139
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             390..407
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(460..478)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      474..521
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(645..664)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta
       61 atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg
      121 tttaacttta agaaggagat atacatatgg aaatggatat tcgctttcgt ggcgatgatc
      181 tggaagcgtt tgaaaaagcc ctgaaagaaa tgattcgtca ggcgcgtaaa tttgcgggca
      241 ccgtgaccta taccctggat ggcaatgatc tggaaattcg tattaccggc gtgccggaac
      301 aggtgcgtaa agaactggcg aaagaagcgg aacgcctggc gaaagaattt aacattaccg
      361 ttacctatac cattcgcggc agcctcgagc accaccacca ccaccactga gatccggctg
      421 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
      481 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
      541 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      601 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      661 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      721 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      781 tggttcacgt a
//
