LOCUS       sequence-64268-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, 
            Baker D
  TITLE     Principles for designing ideal protein structures.
  JOURNAL   Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
  PUBMED    23135467
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-11-8- 8"; volume: "491"; issue: "7423"; pages:
            "222-7"
COMMENT     Sequence Label: Di-II_10_R2x2_769
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             464..481
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(534..552)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      548..595
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(719..738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga
       61 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      121 ttttgtttaa ctttaagaag gagatataca tatgctgctg tatgtgctga ttatcagcaa
      181 cgataaaaaa ctgattgaag aagcgcgtaa aatggccgaa aaagcaaatc tggaactgcg
      241 caccgttaaa accgaagatg aactgaaaaa atatctggaa gaatttcgta aagaaagcca
      301 gaacatcaaa gttctgattc tggttagcaa tgatgaagaa ctggataaag caaaagaact
      361 ggcgcagaaa atggaaatcg atgttcgcac ccgtaaagtt accagcccgg atgaagcaaa
      421 acgctggatt aaagaattca gcgaagaagg cggtagcctc gagcaccacc accaccacca
      481 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      541 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      601 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      721 cctttcgctt tcttcccttc ctttctcgcc acg
//
