LOCUS       sequence-64313-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Trinkle-Mulcahy L, Sleeman JE, Lamond AI
  TITLE     Dynamic targeting of protein phosphatase 1 within the nuclei of 
            living mammalian cells.
  JOURNAL   J Cell Sci. 2001 Dec;114(Pt 23):4219-28.
  PUBMED    11739654
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2001-12"; volume: "114(Pt 23)"; pages: "4219-28"
COMMENT     Sequence Label: pEGFP(N3)-PP1beta
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..130
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     81..101
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      183..192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
       61 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      121 aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc agatctcgag
      181 ccgccaccat ggcggacggg gagctgaacg tggacagcct catcacccgg ctgctggagg
      241 tacgaggatg tcgtccagga aagattgtgc agatgactga agcagaagtt cgaggcttat
      301 gtatcaagtc tcgggagatc tttctcagcc agcctattct tttggaattg gaagcaccgc
      361 tgaaaatttg tggagatatt catggacaat atacagattt actgagatta tttgaatatg
      421 gaggtttccc accagaagcc aactatcttt tcttaggaga ttatgtggac agaggaaagc
      481 agtctttgga aaccatttgt ttgctattgg cttataaaat caaatatcca gagaacttct
      541 ttctcttaag aggaaaccat gagtgtgcta gcatcaatcg catttatgga ttctatgatg
      601 aatgcaaacg aagatttaat attaaattgt ggaagacctt cactgattgt tttaactgtc
      661 tgcccatagc agccattgtg gatgagaaga tcttctgttg tcatg
//
