LOCUS       sequence-64419-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Chong T, Collins JJ 3rd, Brubacher JL, Zarkower D, Newmark PA
  TITLE     A sex-specific transcription factor controls male identity in a 
            simultaneous hermaphrodite.
  JOURNAL   Nat Commun. 2013; 4:1814. doi: 10.1038/ncomms2811.
  PUBMED    23652002
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: " 4:1814. doi"; pages: " 
            10.1038/ncomms2811"
COMMENT     Sequence Label: pTC3-dmd-2
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      420..467
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(479..496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(730..749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaattaata cgactcacta taggtttttt ttttttggaa tctggcgggt aaacaatttc
       61 ttcattttga tacatgacaa aatcattaat ttcctgctcc tcttgcgctt gttgtctccg
      121 caatgcaacc tgagctgcca tcactctttg tcgttccgct attagagcac attttgcaca
      181 gacacaatcc taaaatttat aattagtcaa gaaaatgcaa aatgatttat actttccatc
      241 tacagtatcg cttgtgacct tttaaaggtg aaacatatcc atgattgcgg caacgtgcac
      301 atttaggagt tcgttggtat ttttcaaaat ttcgacaaaa catttgccta ggctgcaagg
      361 tcacttctat agtgtcacct aaatctccct atagtgaggg gtattaggat ccggcgcgcc
      421 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgacg cgtgcttcct
      481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttc
//
