LOCUS       sequence-64457-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP2
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(117..241)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(293..310)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    299..523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(656..675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggagagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
       61 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
      121 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
      181 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
      241 ttcgatcagg gtaatctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
      301 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      361 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      421 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
      481 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
      541 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
      601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
      661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      781 gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa
      841 agta
//
