LOCUS       sequence-64459-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(96..102,103..120,121..126))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(151..275)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(309..353)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(366..386)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(402..425)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(516..540)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    550..568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    571..589
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(591..794)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(620..640)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
       61 tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag
      121 tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag
      181 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
      241 tacgtttctc gttcagcttt tttgtacaaa cttgtttgat gaccgttata gttaatacct
      301 ccacctccgc tgtccatgtg ctggcgttcg aatttagcag cagcggtttc tttcccgccc
      361 ccgccgccct gaaaataaag attctccccg cccccgcctg ccttatcgtc gtcatccttg
      421 taatccatct taagtttaaa cgctagagtc cggaggctgg atcggtcccg gtgtcttcta
      481 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
      541 cgtcgacgat ctctatcact gatagggaga tctctatcac tgatagggag agctctgctt
      601 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      661 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      721 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      781 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      841 aagtcccat
//
