LOCUS       sequence-64595-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ907
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(151..456)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(253..272)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
       61 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
      121 agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc
      181 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac
      241 cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac
      301 cgggtaaagt tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac
      361 catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg
      421 gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg
      481 caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg
      541 ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac
      601 cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac
      661 tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta
      721 tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt
      781 aagccggatc ctctagatta cgccccgcct gccactcatc gcagtactgt tgtaattcat
      841 taagcattct
//
