LOCUS       sequence-64611-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Liu D, Vleugel M, Backer CB, Hori T, Fukagawa T, Cheeseman IM, 
            Lampson MA
  TITLE     Regulated targeting of protein phosphatase 1 to the outer 
            kinetochore by KNL1 opposes Aurora B kinase.
  JOURNAL   J Cell Biol. 2010 Mar 22;188(6):809-20. doi: 10.1083/jcb.201001006. 
            Epub 2010 Mar 15.
  PUBMED    20231380
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201001006"; journalName: "J Cell Biol"; date: 
            "2010-03-22- 22"; volume: "188"; issue: "6"; pages: "809-20"
COMMENT     Sequence Label: mCherry KNL1 wt hardened and RVSF->AAAA
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             305..325
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             338..382
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
       61 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
      121 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
      181 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
      241 catggacgag ctgtacaagt ccggagggga tcggtggagc tccaccggtg gcggccgctc
      301 tagagagaat ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt
      361 cgaacgccag cacatggaca gcggaggtgg aggtactagt ctcgagatgg atggggtgtc
      421 ttcagaggct aatgaagaaa atgacaatat agagagacct gttagaagac ggcattcttc
      481 aatattgaaa cccccaagga gtcctcttca ggacctcaga ggtgggaatg aaacagttca
      541 agagtcaaac gcgttaagga ataagaaaaa ctctcgtgca gccgccgctg cagatactat
      601 aaaggtattc cagacggagt ctcatatgaa aatagtgaga aagtcagaaa tggaagaaac
      661 agaagcagga gaaaatcttc ttttgataca gaataagaaa ttagaagata attactgtga
      721 aattactggg atgaacacat tgctttctgc tcccattcat acccagatgc aacagaagga
      781 gttttcaatt atagaacata cccgtgaaag gaaacatgca aatgaccaga cagtcatttt
      841 ttcagatgaa aaccagatgg acctg
//
