LOCUS       sequence-64725-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, De Las Penas A, Iluore J, 
            Cormack BP
  TITLE     Expression plasmids for use in Candida glabrata.
  JOURNAL   G3 (Bethesda). 2013 Oct 3;3(10):1675-86. doi: 10.1534/g3.113.006908.
  PUBMED    23934995
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.113"; 
            journalName: "G3 (Bethesda)"; date: "2013-10-3- 3"; volume: "3"; 
            issue: "10"; pages: "1675-86"
COMMENT     Sequence Label: pCU-LYS21
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(194..409)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(433..452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     552..574
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(612..630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
       61 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
      121 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
      181 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
      241 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
      301 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
      361 aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg
      421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      481 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      541 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      601 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      661 ttcttagcag gttagactaa catgcaaaaa gtttatattc atttttgtag ttattcttgt
      721 tttcattagg atgattacac actgtggatg tggattaatc gatcattgtt aatttagtgt
      781 agctactacc atttcaaaca aaacgtaaat tcttgcagtc gtactggatc tgtgaatcta
      841 ttagtatata tgaattaaag tagcttgtac atattattct gttgaatcat atcgcagagc
      901 attggttgaa atccaaa
//
