LOCUS       sequence-64740-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Arlotta P, Molyneaux BJ, Chen J, Inoue J, Kominami R, Macklis JD
  TITLE     Neuronal subtype-specific genes that control corticospinal motor 
            neuron development in vivo.
  JOURNAL   Neuron. 2005 Jan 20;45(2):207-21.
  PUBMED    15664173
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2005-01-20"; volume: "45(2)"; pages: "207-21"
COMMENT     Sequence Label: pCRII-Topo Crim1 in situ probe
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(613..632)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(614..632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(639..656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(639..655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(883..902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc tttctcaaga ctgttggttg
      121 ctgcaaaggt agggcctgaa tgagcaggat cacaaaaggc cttgcaaccg agctgccata
      181 ccacaccaga caagcgcatc agaactctcg atagccatcc caggtctaaa gccacaagtt
      241 tctatagagt cacagccgca gtaggcagtg aggaagccag ggagatggaa agcaacagtt
      301 ctcgaaagcg ggttcttgag gatgtattca ctttttttgc tgctgctgtt ttcttccgtg
      361 ccaaccagcc agttcctagg agacacagca ggttgagtag gaacgaggtc acctcttgac
      421 ctgaccactg gagctttgct cgctacgtga caggaagggc aacggcgaag gacaccaggc
      481 atttccaggg gctacacttc attgttcctt gttgttttct tctgtgctat cattggttgt
      541 tcaaagggcg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
      601 gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
      661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      781 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccc
//
