LOCUS       sequence-64746-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orange intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-O-GECO1
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWIKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtcgact catcacgtcg taagtggatt aaggcaggtc acgcagtcag agctataggt
       61 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgt cctgaagagc
      121 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      181 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga cataaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      301 cgccacccca ccggcggcag ggacgagctg tacaagggag gtacaggagg gagtctggtg
      361 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      421 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      541 tgggacatcc tgtcccctca gttcacatac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actactttaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga ggctacgcgt gaccaactga ctgaagagca gatcgcagaa
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
      901 ctggggacgg tgttgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagaa gaatgaatga cacagacagt gaagtggaaa ttagagaagc gttccgcgtg
     1081 tttgataatg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtaa
//
