LOCUS       sequence-64748-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Arlotta P, Molyneaux BJ, Chen J, Inoue J, Kominami R, Macklis JD
  TITLE     Neuronal subtype-specific genes that control corticospinal motor 
            neuron development in vivo.
  JOURNAL   Neuron. 2005 Jan 20;45(2):207-21.
  PUBMED    15664173
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2005-01-20"; volume: "45(2)"; pages: "207-21"
COMMENT     Sequence Label: pCRII-Topo S100a10 in situ probe
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(427..446)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(428..446)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(453..470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(453..469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..484)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(697..716)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     907..928
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accganctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttccactagt gatagaaagc
      121 tctggaagcc cactttgcca tctcggcact ggtccaggtc cttcattatt ttgtccacag
      181 ccagagggtc cttttgattt tccaaaaacc cagggaactc ccgttccatg agcactctca
      241 ggtcctccct tgtcaagtgg tctttgtcgc ctgcaaacct gtgaaacgta agcatcatgg
      301 tttccatggc gtgctccatt tgggatggca ttttgaagag tctgtcgaaa cctgggcaag
      361 ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
      421 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
      481 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      541 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      601 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
      661 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
      721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
      781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
      841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
      901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccc
//
