LOCUS       sequence-64771-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, 
            Macklis JD
  TITLE     Novel subtype-specific genes identify distinct subpopulations of 
            callosal projection neurons.
  JOURNAL   J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 
            10.1523/JNEUROSCI.6108-08.2009.
  PUBMED    19793993
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6108-08"; journalName: "J Neurosci"; date: 
            "2009-09-30- 30"; volume: "29"; issue: "39"; pages: "12343-54"
COMMENT     Sequence Label: pCRII-Topo Nectin-3 in situ probe
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(585..604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(586..604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(611..628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(611..627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(620..642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(855..874)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttcagtgaaa actgtaaagc
      121 agctcagata cattgaaccc aggttttaca ttttcatttg tcagatcaga atataaaaag
      181 taagctcgaa atcaacttga aaaagcttat tcttcctcaa gaaaaagtat gaacaatctg
      241 aaaatggtca actagtgtga atgaaacctt gtacatagtg aagtcgcgtc agtggctgtt
      301 agacatacca ctccctcctg gaaattacgg agccatctac atgagaaacc aaaccatctt
      361 cactttcatt gtagcgttca tcactgacaa acttcattcc catttttaga tcttcatagt
      421 aatccatcgg tctttcaaac ctagtgaggt tctctacatt attccactga gttttctcag
      481 gctcctctaa gtagtctttg cggatcaggt tgtttaaggg cgaattctgc agatatccat
      541 cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt
      601 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      661 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      721 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacg
//
