LOCUS       sequence-64776-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Balskus EP, Walsh CT
  TITLE     The genetic and molecular basis for sunscreen biosynthesis in 
            cyanobacteria.
  JOURNAL   Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637.
            Epub 2010 Sep 2.
  PUBMED    20813918
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1193637"; journalName: "Science"; date: 
            "2010-09-24- 24"; volume: "329"; issue: "5999"; pages: "1653-6"
COMMENT     Sequence Label: pET28b-Ava3856
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcacaa tcccttcccc tttcttccgc acctgctaca
      121 ccgtctcttc cttcccagac gaaaatagcc gcaattatcc aaaatatctg cactttggct
      181 ttgttattac tagcattgcc cattaatgcc accattgttt ttatatcctt gttagtcttc
      241 cgaccgcaaa aggtcaaagc agcaaacccc caaaccattc ttatcagtgg cggtaagatg
      301 accaaagctt tacaactagc aaggtcattc cacgcggctg gacatagagt tgtcttggtg
      361 gaaacccata aatactggtt gactggtcat cgtttttccc aagcagtgga taagttttac
      421 acagtccccg caccccagga caatccccaa gcttacattc aggctttggt agatatcgtc
      481 aaacaagaaa acatcgatgt ttatattccc gtcaccagtc cagtgggtag ctactacgac
      541 tcattagcca aaccagagtt atcccattat tgcgaagtgt ttcactttga cgcagatatt
      601 acccaaatgt tggatgataa atttgcgttg acacaaaaag cgcgatcgct tggtttatca
      661 gtacccaaat cctttaaaat tacctcacca gaacaagtca tcaacttcga tttttctgga
      721 gagacacgta aatacatcct caaaagcatt ccctacgact cagtgcggcg gttggactta
      781 accaaactcc cctgtgctac tccagaggaa acagcagcat tcgtcagaag tttgccaatt
      841 actcccgaaa aaccgtggat tatgcaggaa tttatccccg gtaag
//
