LOCUS       sequence-64778-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: pMito-iRFP670
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..112
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcatg tccgtcctga cgccgctgct
       61 gctgcggggc ttgacaggct cggcccggcg gctcccagtg ccgcgcgcca agatccattc
      121 gttgggggat ccaccggtcg ccaccatggc gcgtaaggtc gatctcacct cctgcgatcg
      181 cgagccgatc cacatccccg gcagcattca gccgtgcggc tgcctgctag cctgcgacgc
      241 gcaggcggtg cggatcacgc gcattacgga aaatgccggc gcgttctttg gacgcgaaac
      301 tccgcgggtc ggtgagctac tcgccgatta cttcggcgag accgaagccc atgcgctgcg
      361 caacgcactg gcgcagtcct ccgatccaaa gcgaccggcg ctgatcttcg gttggcgcga
      421 cggcctgacc ggccgcacct tcgacatctc actgcatcgc catgacggta catcgatcat
      481 cgagttcgag cctgcggcgg ccgaacaggc cgacaatccg ctgcggctga cgcggcagat
      541 catcgcgcgc accaaagaac tgaagtcgct cgaagagatg gccgcacggg tgccgcgcta
      601 tctgcaggcg atgctcggct atcaccgcgt gatgttgtac cgcttcgcgg acgacggctc
      661 cgggatggtg atcggcgagg cgaagcgcag cgacctcgag agctttctcg gtcagcactt
      721 tccggcgtcg ctggtcccgc agcaggcgcg gctactgtac ttgaagaacg cgatccgcgt
      781 ggtctcggat tcgcgcggca tcagcagccg gatcgtgccc gagcacgacg cctccggcgc
      841 cgcgctcgat ctgtcgttcg cgcacctgcg cagcatctcg ccctgccatc tcgaatttct
      901 gcggaacatg ggcgtcagcg cctcgatgtc gctgtcgatc atcattgacg gcacgctatg
      961 gggattgatc atctgtcatc attacgagcc gcgtgcc
//
