LOCUS       sequence-64780-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Abbate J, Lacayo JC, Prichard M, Pari G, McVoy MA
  TITLE     Bifunctional protein conferring enhanced green fluorescence and 
            puromycin resistance.
  JOURNAL   Biotechniques. 2001 Aug;31(2):336-40.
  PUBMED    11515370
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "2001-08"; volume: "31(2)"; pages: "336-40"
COMMENT     Sequence Label: pEGFP-puro
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..652
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(56..75)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     552..572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    782..903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(819..838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     873..892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtccgg actcagatct aggagacgac cttccatgac
       61 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
      121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
      181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
      241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
      301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
      361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
      421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
      481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
      541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
      601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cgggatccac
      661 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
      721 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      781 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      841 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      901 ttaacgcgat caagcttatc gatacc
//
