LOCUS       sequence-64786-e       10426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, 
            Christoforou N, Epstein JA, Gearhart JD
  TITLE     Optimization of direct fibroblast reprogramming to cardiomyocytes 
            using calcium activity as a functional measure of success.
  JOURNAL   J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 
            10.1016/j.yjmcc.2013.04.004.
  PUBMED    23591016
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Cell 
            Cardiol."; date: "2013-04-13"; volume: "60C:97-106. doi"; pages: " 
            10.1016/j.yjmcc.2013.04.004"
COMMENT     Sequence Label: TroponinT-Gateway
FEATURES             Location/Qualifiers
     source          1..10426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3220..3239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3220..3238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3270..3394
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3419..3424,3425..3442,3443..3449)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3503..4162
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3569..3588)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4504..4809
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4688..4707
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4850..4974)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5000..5041
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5051..5068
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5118..5706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5171..5191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5589..5600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5709..5725)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5710..5726)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6231..6411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6437..6454)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6443..6667
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6713..7141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6800..6819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7010..7031
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7150..7170)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7155..7484
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7335..7470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7397..7416
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7532..7579
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7598..7972
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8102..8223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8139..8158)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8193..8212
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8272..8288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8272..8288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8285..8307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8296..8312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8320..8326,8327..8344,8345..8350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8365..8386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8503..8520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8674..9262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8754..8773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9433..10224,10225..10293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10056..10075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10294..10398)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtcatggag aagacccacc ttgcagatgt cctcactggg
     2641 gctggcagag ccggcaacct gcctaaggct gctcagtcca ttaggagcca gtagcctgga
     2701 agatgtcttt acccccagca tcagttcaag tggagcagca cataactctt gccctctgcc
     2761 ttccaagatt ctggtgctga gacttatgga gtgtcttgga ggttgccttc tgccccccaa
     2821 ccctgctccc agctggccct cccaggcctg ggttgctggc ctctgcttta tcaggattct
     2881 caagagggac agctggttta tgttgcatga ctgttccctg catatctgct ctggttttaa
     2941 atagcttatc tgagcagctg gaggaccaca tgggcttata tggcgtgggg tacatgttcc
     3001 tgtagccttg tccctggcac ctgccaaaat agcagccaac accccccacc cccaccgcca
     3061 tccccctgcc ccacccgtcc cctgtcgcac attcctccct ccgcagggct ggctcaccag
     3121 gccccagccc acatgcctgc ttaaagccct ctccatcctc tgcctcaccc agtccccgct
     3181 gagactgagc agacgcctcc aggatccgcc accctcgagt aatacgactc actataggga
     3241 gacccaagct ggctaggtaa gcttgatcaa caagtttgta caaaaaagct gaacgagaaa
     3301 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     3361 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     3421 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt
     3481 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     3541 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     3601 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     3661 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     3721 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     3781 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     3841 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     3901 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     3961 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     4021 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     4081 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     4141 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     4201 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     4261 tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     4321 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     4381 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     4441 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt
     4501 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     4561 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     4621 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     4681 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     4741 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     4801 ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc atagtgactg
     4861 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     4921 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatct
     4981 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5041 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcaattcg
     5101 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5161 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5221 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5281 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5341 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5401 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5461 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5521 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5581 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5641 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5701 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     5761 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5821 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5881 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5941 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     6001 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     6061 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     6121 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     6181 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     6241 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6301 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6361 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     6421 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6481 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6541 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6601 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6661 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7021 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7141 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7201 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7501 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     7561 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     7621 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     7681 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     7741 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     7801 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7861 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7921 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7981 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8041 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8101 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8161 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8221 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     8281 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8341 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8461 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8521 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8581 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8641 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8701 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8761 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8821 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8881 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8941 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9001 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9061 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9121 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9181 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9241 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9301 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9361 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9421 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9481 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9541 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9601 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9661 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9721 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9781 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9841 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9901 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9961 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10021 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10081 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10141 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10201 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10261 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10321 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10381 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
