LOCUS       sequence-64976-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK5
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      458..467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(712..732)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(757..774)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             785..807
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    822..846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(846..865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcggatccgt atccaccttt actgtcacaa
      121 gaaaatagag tcaatagctg tgcagagttg aagaaaaacg tcctctggtg ttccctctgc
      181 atttatcttg tgtagctgtg tttttgtctc gtagtaggcg atcacgggga tggacgctcg
      241 gtagtaggct tctaggcgct tggcgatggt cttggtggtg tcgtccacag gcaggctgct
      301 ccggctcatt tggagaaggc ggttggtcat ggtgtctgcc gagcagtcca tacagatcac
      361 caactgtggg tctccaatcc tgcgtccgaa ctcttcccct tgcttcacct cccgaggata
      421 gccgtcaatc aggaagcccc tggtgtcccc gaggctggcc accatggcct ccttcaggag
      481 ctccaaaacg atgcctgagg gcaccaggtc tccacgttcc ataatgtctc tgatcaattt
      541 gcttctttca gattctgatg ccagttcctc acgcaggagc tcgccagttg agagatgtgt
      601 aaatccatat ttttccacca gcttttcaca ctgtgtgcct ttgccagagc caggaccacc
      661 aattatgaaa ataattttac actttctcaa atcttccatg aaacctccca tggattggaa
      721 gtacaggttc tcggtaccca gatctacacc agaagaatga tgatgatgat ggtgcatatg
      781 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      841 aattccccta tagtgagtcg tattaatttc gcgggatcga gatcgatctc gatcctctac
      901 gccggacgca tcgtggccgg catca
//
