LOCUS       sequence-64977-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ERAP1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..89)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(90..119)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(120..140)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctgattat gatcctctag tacttctcga caagcttgtc gacggagctc gaattcggat
       61 cctcacttat cgtcgtcatc cttgtaatca tggtggtgat ggtgatggtg atgatggtgc
      121 gattggaagt agaggttctc tgccatacgt tcaagctttt cactttgcag ccacactctg
      181 attttatcaa aattcttatc catccaaccg atgttttctt caatggtttc aattgtctgt
      241 tggacacaac ggagctgaga accattttct ttcaaagagc tgaagaatcc ttttacctct
      301 tcaagccgtg ttcttgtgga gaattgattt gttgtaccca ttaccatgtg ggctatggaa
      361 gatgagccaa gttcaaactt ttgtacaagt ttgttccagt ttttcctcag aaattgccag
      421 gccagtgggt atcctactgg gttcctgcca atgagtgtaa gaatttgtgg aaactcctga
      481 gtttttattt tatctccctt aaagctttca tctagtagcc attgaagctt ttccttattt
      541 tgggttctgc agagggcaaa ttcaatttgg cttttctcag tactggacaa agaaaactga
      601 tatttactat aaagaaaatc ccagccttct gtgctctggg cccccacagc aaacactgcc
      661 aaggtcacgt cgacaggcag gctcaagttt ccattggatt ccttccactt tctgaaatag
      721 ccttctgccc tctgtacgca cggctgatag ttgtgcacac aggcgaggag tagtagttga
      781 ctccgcagca ttcgctctga gactgagccc tcgtctgtcc atgtctgctt atcaatgagg
      841 tcccttagca gcctgatgag gaaggccttg aattgagttt ccacttcat
//
