LOCUS       sequence-65000-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Yanagiya A, Svitkin YV, Shibata S, Mikami S, Imataka H, Sonenberg N
  TITLE     Requirement of RNA binding of mammalian eukaryotic translation 
            initiation factor 4GI (eIF4GI) for efficient interaction of eIF4E 
            with the mRNA cap.
  JOURNAL   Mol Cell Biol. 2009 Mar;29(6):1661-9. doi: 10.1128/MCB.01187-08. 
            Epub 2008 Dec 29.
  PUBMED    19114555
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01187-08"; journalName: "Mol Cell Biol"; date: 
            "2009-03"; volume: "29"; issue: "6"; pages: "1661-9"
COMMENT     Sequence Label: pcDNA3 HA eIF4GI (1-1599)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccac catgggatat ccatacgacg tcccagacta
      121 tgctggaatg aacaaagctc cacagtccac aggcccccca cccgccccat cccccggact
      181 cccacagcca gcgtttcccc cggggcagac agcgccggtg gtgttcagta cgccacaagc
      241 gacacaaatg aacacgcctt ctcagccccg ccagcacttc taccctagcc gggcccagcc
      301 cccgagcagt gcagcctccc gagtgcagag tgcagcccct gcccgccctg gcccagctgc
      361 ccatgtctac cctgctggat cccaagtaat gatgatccct tcccagatct cctacccagc
      421 ctcccagggg gcctactaca tccctggaca ggggcgttcc acatacgttg tcccgacaca
      481 gcagtaccct gtgcagccag gagccccagg cttctatcca ggtgcaagcc ctacagaatt
      541 tgggacctac gctggcgcct actatccagc ccaaggggtg cagcagtttc ccactggcgt
      601 ggcccccgcc ccagttttga tgaaccagcc accccagatt gctcccaaga gggagcgtaa
      661 gacgatccga attcgagatc caaaccaagg aggaaaggat atcacagagg agatcatgtc
      721 tggggcccgc actgcctcca cacccacccc tccccagacg ggaggcggtc tggagcctca
      781 agctaatggg gagacgcccc aggttgctgt cattgtccgg ccagatgacc ggtcacaggg
      841 agcaatcatt gctgaccggc cagggctgcc tgg
//
