LOCUS       sequence-65037-e        9182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pCasper4-QF#7-hsp70
FEATURES             Location/Qualifiers
     source          1..9182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1..31,32..586)
                     /label=P element 5' end
     gene            complement(597..4725)
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4698..4716
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     misc_feature    join(6190..6391,6392..6422)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(6959..6978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7078..7100
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7138..7156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7224..7328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7329..7397,7398..8189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7547..7566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8360..8948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8849..8868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaaatgcaa ctgaaggcgg acattgacgc
     4441 tacgtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtaca tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatatccag tgcagtaaaa aaaaaaaatg ttttttttat
     4681 ctactttccg caaaaatggg ttttattaac ttacatacat actagaattc ggtacccgcc
     4741 cggggatcag atccgcggcc gcataggcca ctagtggatc tggatcctct agacaacatg
     4801 ccacccaagc gcaaaacgct taacgctgcg gctgaggcta acgctcatgc cgacggacac
     4861 gccgacggaa acgccgacgg acacgtggcc aatacggccg cgtcctcgaa taatgcgagg
     4921 ttcgctgatc tcactaacat cgatactccg ggtctgggac ccacaactac gaccctgctc
     4981 gtggaaccag cacgctcaaa gcgtcaacga gtgtcccgcg catgcgacca gtgccgtgca
     5041 gcccgagaga aatgcgacgg aatacagcct gcgtgtttcc cgtgcgtttc ccagggaagg
     5101 tcctgcactt atcaggcttc gccgaaaaag aggggagttc aaaccggtta tattcgtacg
     5161 ctggagctcg ccctcgcctg gatgtttgaa aatgtcgcgc gttccgaaga tgccttgcat
     5221 aacctcctcg tccgtgacgc cggacaagga tcagctctgc tcgttggtaa agattcgccg
     5281 gctgccgagc gactccatgc ccgttgggct actagccgtg tcaataagag cattacccgc
     5341 ctcctccgtc agttggagct ccctcctacc gccacggcta cggcctcgat aatgccgcac
     5401 gtgatggagc agcctctcag taccagcatt aaccccgtca acgaccgctt caacggtatt
     5461 cccaacccca ctccgtataa ctccgatgca gctctcgatg ctatcactca gaccaacgat
     5521 tatggaagcg taaatacaca tggtatcctc tctacttacc cgccaccggc tacgcacctt
     5581 aatgaagctt ccgtcgctct cgctcccggt ggcgcccccc cccgaccgcc tcctccgtat
     5641 gttgacagca cgaccaatca cccgccgtac cactcgaatc tggttccaat ggcgaacttt
     5701 ggttactcga ccgttgatta cgatgccatg gttgacgatt tggctagcat tgaatacacg
     5761 gacgctgtgg atgtcgaccc acagtttatg accaatctgg gattcgttcc tggatgtaac
     5821 ttctccgaca ttaatacata cgaacagtga tgacgtctat cgataccgtc gactaaagcc
     5881 aaatagaaaa ttattcagtt cctggcttaa gtttttaaaa gtgatattat ttatttggtt
     5941 gtaaccaacc aaaagaatgt aaataactaa tacataatta tgttagtttt aagttagcaa
     6001 caaattgatt ttagctatat tagctacttg gttaataaat agaatatatt tatttaaaga
     6061 taattgcgtt tttattgtca gggagtgagt ttgcttaaaa actcgtttag atcctctaga
     6121 gtcgacctcg aacgttaacg ttaacgtaac gttaactcga ggcctcgagg tcgacctgca
     6181 gccaagcttt gcgtactcgc aaattattaa aaataaaact ttaaaaataa tttcgtctaa
     6241 ttaatattat gagttaattc aaaccccacg gacatgctaa gggttaatca acaatcatat
     6301 cgctgtctca ctcagactca atacgacact cagaatacta ttcctttcac tcgcacttat
     6361 tgcaagcata cgttaagtgg atgtctcttg ccgacgggac caccttatgt tatttcatca
     6421 tggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     6481 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc
     6541 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     6601 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     6661 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     6721 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     6781 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     6841 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     6901 gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg catatggtgc actctcagta
     6961 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     7021 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     7081 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     7141 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     7201 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7261 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7321 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7381 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7441 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7501 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7561 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7621 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7681 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7741 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7801 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7861 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7921 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7981 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8041 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8101 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8161 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     8221 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8281 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8341 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8401 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8461 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     8521 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8581 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8641 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8701 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     8761 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8821 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8881 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8941 tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     9001 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     9061 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     9121 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     9181 cc
//
