LOCUS       sequence-65103-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A
  TITLE     Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end 
            rule pathway.
  JOURNAL   J Biol Chem. 2008 Aug 29. 283(35):24011-28.
  PUBMED    18566452
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Aug 29. 283(35):24011-28."
COMMENT     Sequence Label: UPR pBAM Ura3
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(60..340)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(361..380)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     480..502
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(540..558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat aacagacata
       61 ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag tcaccaatgc
      121 cctccctctt ggccctctcc ttttcttttt tcgaccgaat taattcttaa tcggcaaaaa
      181 aagaaaagct ccggatcaag attgtacgta aggtgacaag ctatttttca ataaagaata
      241 tcttccacta ctgccatctg gcgtcataac tgcaaagtac acatatatta cgatgctgtt
      301 ctattaaatg cttcctatat tatatatata gtaatgtcgt gatctatggt gcactctcag
      361 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
      421 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      481 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
      541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgga
      601 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
      661 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
      721 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
      781 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
      841 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
      901 aagatgaaac aattcggcat taatacctga gagcaga
//
