LOCUS       sequence-65140-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Grp
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(611..630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(612..630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(637..654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(637..653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(646..668)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttacgcactc tcagcctagt
      121 cgagagctct gagggtttcc ctccaggacc atgcgcggct cggagctctc gctcttgctg
      181 ttggctctgg tcctctgcca ggcgccccgg gggccagctg ccccggtgtc gacaggcgca
      241 ggtggaggca cggtcctggc taagatgtat ccgcgcggca gtcactgggc tgtgggacac
      301 ttaatgggaa aaaagagcac ggatgaatcc ccgtccctgt atgcggctga cagagacggc
      361 ctgaaggagc agctgagggg gtacgtccgc tgggaagaag ctgcaaggga tttgctggac
      421 ctcctagaag ctgctgggaa ccaaagccac cagccacctc agcatccgcc tctcagtctc
      481 cagcctactt gggatcccga ggacggcagc tactttaacg acgttcaaac cgctaagttg
      541 gaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
      601 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
      661 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      721 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      781 gaatggcgaa tggacgcgcc ctgtagcggc gcattaa
//
