LOCUS       sequence-65276-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR
  TITLE     Dynamics and memory of heterochromatin in living cells.
  JOURNAL   Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. 
            Epub 2012 Jun 14.
  PUBMED    22704655
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.03.052"; journalName: "Cell"; date: 
            "2012-06-22- 22"; volume: "149"; issue: "7"; pages: "1447-60"
COMMENT     Sequence Label: N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A>
            HP1aCS...
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(277..543)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="MWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSF
                     NQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     protein_bind    560..584
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gacgtggtag tacaaatccc aagcctgtgt caaatctttc acgttgccgc tcttcatata
       61 tttgcgacac cattcctgag cttccatcag gtccctgccg taagcttggt tgaaactagt
      121 ctctttgagg gtttgaggtc ctctctccat catggcgtgg agaggttcga ggacctcgaa
      181 cattcccttg acattgcgct caccgaaata aagtctgcta gcttcctcca atccctcgtg
      241 ccacattgat ccaccgccgc tacctccgcc gccgaactgc tttgagattc gtcggaacac
      301 atgataatag aggtcccagg cttgggtgag gtccttgaca ttccctgatt tcatgtactt
      361 cctgcaccac tcttgggcct ccattaaatc tcgaccatag gcctgattaa aggatgtttc
      421 cttcagagtc tgggggcccc gttccatcat agcatgcaag ggctccagca cctcaaacat
      481 gcctttcacg ttcctttccc caaagtacaa acgagatgcc tcttccaggc cttcatgcca
      541 catagggccc tctagatcaa ccactttgta caagaaagct gggtcgaatt cgcccttgct
      601 cttcgcgctt tctttttctt tgttttccgc atcctctgga tatgcgtgcc acgtcagtct
      661 ctcttcataa aatgctatca caatctgtgg acacttcacg ttagcttctt ttgcaagaac
      721 caggtcagct tcatctgtgt ctttccattt cattaagaac attaagtcac cgcaggaatc
      781 tgttgctccg atgatctttt ctggttccag tcctctctca aagccccgag cgatatcatt
      841 gctttgctct ctcttttttt tagatttaat atcatca
//
