LOCUS       sequence-65429-e        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-PLK1
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     547..566
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        547..565
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatccatgg aacgagctat acaaggaaac gacgctaggg aacaagctaa cagtgaacgt
       61 tgggatggag gatcaggagg taccacttct cccttcaaac ttcctgacga aagtccgagt
      121 tggactgagt ggcggctaca taacgatgag acgaattcga atcaagataa tccccttggt
      181 ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga gatatctcag atacgacagg
      241 acggaagctt cactgcacag agtccttgga tcttggacgg gagattcggt taactatgca
      301 gcatctcgat ttttcggttt cgaccagatc ggatgtacct atagtattcg gtttcgagga
      361 gttagtatca ccgtttctgg agggtcgcga actcttcagc atctctgtga gatggcaatt
      421 cggtctaagc aagaactgct acagcttgcc ccaatcgaag tggaaagtaa tgtatcaaga
      481 ggatgccctg aaggtactca aaccttcgaa aaagaaagcg agcaccacca ccaccaccac
      541 tgaatctaat acgactcact ataggggagc tccggcggcg gctccaccgg cgaaagagat
      601 cccggaggtc ctagtggacc cacgcagccg gcggcgctat gtgcggggcc gctttttggg
      661 caagggcggc tttgccaagt gcttcgagat ctcggacgcg gacaccaagg aggtgttcgc
      721 gggcaagatt gtgcctaagt ctctgctgct caagccgcac cagagggaga agatgtccat
      781 ggaaatatcc attcaccgca gcctcgccca ccagcacgtc gtaggattcc acggcttttt
      841 cgaggacaac gacttcgtgt tcgtggtgtt gctcgagtct agagggcccg tttaaacccg
      901 ctgatcagcg tcgaccaaca ccacgaacac gaagtcgttg tcctcgaaaa agccgtggaa
      961 tcctacgacg tgctggtggg cgaggctgcg gtgaatggat atttccatgg acatcttctc
     1021 cctctggtgc ggcttgagca gcagagactt aggcacaatc ttgcccgcga acacctcctt
     1081 ggtgtccgcg tccgagatct cgaagcactt ggcaaagccg cccttgccca aaaagcggcc
     1141 ccgcacatag cgccgccggc tgcgtgggtc cactaggacc tccgggatct ctttcgccgg
     1201 tggagccgcc gccggcggcc gc
//
