LOCUS       sequence-65520-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Zhang J, Schulze KL, Hiesinger PR, Suyama K, Wang S, Fish M, Acar M,
            Hoskins RA, Bellen HJ, Scott MP
  TITLE     Thirty-one flavors of Drosophila rab proteins.
  JOURNAL   Genetics. 2007 Jun;176(2):1307-22. Epub 2007 Apr 3.
  PUBMED    17409086
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2007-06"; volume: "176(2)"; pages: "1307-22. Epub 2007 Apr 3"
COMMENT     Sequence Label: pUASP YFP Rab9Fb WT
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..375
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     312..333
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
       61 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      121 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      181 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      241 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      301 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      361 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac
      421 ggtaccgcgg gcccgggatc atcaacaagt ttgtacaaaa aagcaggctt aatgagccaa
      481 tacgactatc tgtttaagat tctcgtcctg ggtgacatcg gcgtgggcaa atcctgcctg
      541 ctgatgcgtt tctcggataa ccgattcacc gagaaacacg tgtgcacggt gggcatggat
      601 ttcagggtgc gcaacgtgga gttggccggt cggatggtta tgctgcagat ctgggatacc
      661 gccggcgatg agcgctttaa gtcgctgctg ccatcctatt atcgcggtgc ccatggcata
      721 ctgctcgttt acgacataac gtcgtccaaa agtttccgaa acatcgatgg ctggctgaag
      781 gagatacggc gcatgtcttc ggaaagtgtg aacgtcatgc tggtgggcaa caagtgtgat
      841 gatctggata atcgtcaggt gcgcatggag cagggtttca actatgccaa tcatcgggca
      901 ctcggctttt atgaggtttc cgccaagagt ggcgcgaatg taaatgacgt attcaacatg
      961 ttgtcagttg gcatatacaa tcgtcttgtg attcataccc caaatc
//
