LOCUS       sequence-65798-e        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..72
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     768..787
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             811..1254
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 atggtcgact catcacgtcg taagtggaat aaggcaggtc acgcagttag agctataggt
       61 cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc
      121 gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc
      181 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
      241 gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc
      301 cgccactcca ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg
      361 agcaagggcg tggaggataa catggccatc gtcaaggagt tcatgcgctt caagacgcac
      421 atagagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      481 tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      541 tgggacatcc tgtcccctca gattatgtac ggctccaagg cctacattaa gcacccagcc
      601 gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg
      661 aacttcgagg acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgta
      721 ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
      781 aagaagacca tgggctggga gcctacgcgt gaccaactga ctgaagagca gatcgcagaa
      841 tttaaagagg ctttctccct atttgacaag gacggggatg ggacgataac aaccaaggag
      901 ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg
      961 atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg
     1021 atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttagagaagc gttccgcgtg
     1081 tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac
     1141 cttggagaga agttaacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat
     1201 ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa gtaa
//
