LOCUS       sequence-66186-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL69
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatgga gacttccctc ccccagcacc cccatgaccc gctccagatt
       61 aacccgggcc acgagatagt cgcgccgtgc ccgggccagg ttcccccggg cctgacgcag
      121 gttcagttcg gcgtcgtcca cctccagccg aatcttgacc cccagctcga atcccttttc
      181 ggccatctgc agaaggcgct ccgcctggac caccgtaccg gacagggcct tgacaatctc
      241 ctcggactcg cgaacagcat tgaccgcatc gcggatttcc agggcaaccg actccaggag
      301 tttagcctcg tcgatctgca tgcttcgccg ttcgctctcg gcctgggcca ccttgccccg
      361 ggtcttgagg ccatcgaaca ggggaaaact caactgaagg cccacggtcc atatctggcc
      421 gctcccggaa ccgtccccca cctccaggtg ccgccatccg tatttccccg tcaggtccag
      481 acgcggcttg tcgtcggcgt cagccacctt caccagttca ccggcaatgg cgaggcgatg
      541 cttgatctcc ttcagctcgg gacgtttatc ccgggccact gcgagggctt tgtcgtagga
      601 gggataggta gtcagcacag tctccagcga gccggtcaca tccacctcac cctccagggc
      661 caggaggaag gagagccggt cccgggccac gcggacactg ttgccggccc ggatcacgtc
      721 gggcatggca ttttgcaccg caacatccgc tgccaggacg tcgtaatcgg tggccacgcc
      781 ggccgcatac ctgcgctggg cctcatcctg gtgccgcacc ttctgcgcaa
//
