LOCUS       sequence-66212-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, 
            Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
  TITLE     A Tumor suppressor complex with GAP activity for the Rag GTPases 
            that signal amino acid sufficiency to mTORC1.
  JOURNAL   Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
  PUBMED    23723238
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-05-31- 31"; volume: "340"; 
            issue: "6136"; pages: "1100-6"
COMMENT     Sequence Label: Flag Depdc5 pLJM1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtgccacca
       61 tggactacaa ggatgacgat gacaagggtg gtggtggtgg tgcgtcgacg atgagaacaa
      121 caaaggtcta caaactcgtc atccacaaga agggctttgg gggcagtgat gatgagctag
      181 ttgtgaaccc caaagtgttc cctcacatca agcttggaga cattgtagag attgcacacc
      241 ccaacgatga atacagccct ctgcttttgc aggtcaagtc tcttaaggaa gatttacaga
      301 aggaaactat cagtgtggac cagactgtga ctcaagtgtt ccggctgaga ccttatcagg
      361 atgtctatgt taatgtcgta gaccctaagg atgtgaccct tgacctagtg gaattaactt
      421 ttaaggatca gtatattggc cgtggggata tgtggcgact aaagaaaagt ttggtcagca
      481 catgtgccta tatcacccag aaggtggagt ttgctggcat cagagcacag gctggtgaac
      541 tgtgggttaa gaatgagaag gtcatgtgtg gctacatcag tgaagatacc agggtggtgt
      601 ttcgttctac gtcggctatg gtttacatat ttattcagat gagctgtgaa atgtgggatt
      661 ttgatattta tggggatttg tattttgaga aagctgtgaa tggtttcctt gctgatctat
      721 ttaccaagtg gaaggagaag aactgtagtc atgaagtgac agtggtcctg ttttctagaa
      781 ctttctatga tgcaaaatct gttgatgaat ttcctgaaat aaaccgagcc tcaattcgac
      841 aggatcacaa ggggagattc tatgaagact tttacaaagt ggtggtgcag aatgagagaa
      901 gagaagaatg g
//
