LOCUS       sequence-66233-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson 
            D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W
  TITLE     Conditional control of gene function by an invertible gene trap in 
            zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 
            Aug 20.
  PUBMED    22908272
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15389-94. Epub 2012 Aug 20"
COMMENT     Sequence Label: pFT1.2
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(221..268)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(293..326)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    359..392
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    439..472
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg
      121 ttcttctctt cttccgaccg cgaagagttt gtcgacctga aaaaaaaaag ggaagagaga
      181 gacacgtcag aaacacacac acactccgga ttagtctcga gaagttccta tactttctag
      241 agaataggaa cttcggaata ggaacttcac acacacacac acacacacac acgaagttcc
      301 tatactattt gaagaatagg aacttcggaa taggaacttc attaccctgt tatccctaat
      361 aacttcgtat aatgtatgct atacgaagtt atccaagcat caccatcgac cctctagtcc
      421 agatctcacc atcgacccat aacttcgtat aatgtgtact atacgaagtt atgggcccat
      481 ctggcctgtg tttcagacac cagggagtct ctgctcacgt ttcctgctat ttgcagcctc
      541 tctatcaaga ctaatacacc tcttcccgca tcggctgcct gtgagaggct tttcagcact
      601 gcaggattgc ttttcagccc caaaagagct aggcttgaca ctaacaattt tgagaatcag
      661 cttctactga agttaaatct gaggttttac aactttgagt agcgtgtact ggcattagat
      721 tgtctgtctt atagtttgat aattaaatac aaacagttct aaagcaggat aaaaccttgt
      781 atgcatttca tttaatgttt tttgagatta aaagcttaaa caagaatctc tagttttctt
      841 tcttgctttt acttttactt ccttaatact caag
//
