LOCUS       sequence-66353-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hM3D (Gq) neuronal activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL
  TITLE     Evolving the lock to fit the key to create a family of G 
            protein-coupled receptors potently activated by an inert ligand.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 
            2.
  PUBMED    17360345
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-03-20"; volume: "104(12)"; pages: 
            "5163-8. Epub 2007 Mar 2"
COMMENT     Sequence Label: pcDNA5/FRT-HA-hM3D(Gq)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccgagctc ggatccacca tgtacccata
      121 cgatgttcca gattacgcta tgaccttgca caataacagt acaacctcgc ctttgtttcc
      181 aaacatcagc tcctcctgga tacacagccc ctccgatgca gggctgcccc cgggaaccgt
      241 cactcatttc ggcagctaca atgtttctcg agcagctggc aatttctcct ctccagacgg
      301 taccaccgat gaccctctgg gaggtcatac cgtctggcaa gtggtcttca tcgctttctt
      361 aacgggcatc ctggccttgg tgaccatcat cggcaacatc ctggtaattg tgtcatttaa
      421 ggtcaacaag cagctgaaga cggtcaacaa ctacttcctc ttaagcctgg cctgtgccga
      481 tctgattatc ggggtcattt caatgaatct gtttacgacc tacatcatca tgaatcgatg
      541 ggccttaggg aacttggcct gtgacctctg gcttgccatt gactgcgtag ccagcaatgc
      601 ctctgttatg aatcttctgg tcatcagctt tgacagatac ttttccatca cgaggccgct
      661 cacgtaccga gccaaacgaa caacaaagag agccggtgtg atgatcggtc tggcttgggt
      721 catctccttt gtcctttggg ctcctgccat cttgttctgg caatactttg ttggaaagag
      781 aactgtgcct ccgggagagt gcttcattca gttcctcagt gagcccacca ttacttttgg
      841 cacagccatc gctggttttt atatgcctgt caccattatg actattttat actggaggat
      901 ctata
//
