LOCUS       sequence-66371-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Marx CJ, Lidstrom ME
  TITLE     Development of improved versatile broad-host-range vectors for use 
            in methylotrophs and other Gram-negative bacteria.
  JOURNAL   Microbiology. 2001 Aug;147(Pt 8):2065-75.
  PUBMED    11495985
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2001-08"; volume: "147(Pt 8)"; pages: 
            "2065-75"
COMMENT     Sequence Label: pCM130
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1..18)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      171..257
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      349..376
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
       61 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
      121 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
      181 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      241 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
      301 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
      361 tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct acaagccatc
      421 cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcaggg aattctagat
      481 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttcaggagg
      541 tgacgtcatg aacaaaggtg taatgcgacc gggccatgtg cagctgcgtg tactggacat
      601 gagcaaggcc ctggaacact acgtcgagtt gctgggcctg atcgagatgg accgtgacga
      661 ccagggccgt gtctatctga aggcttggac cgaagtggat aagttttccc tggtgctacg
      721 cgaggctgac gagccgggca tggattttat gggtttcaag gttgtggatg aggatgctct
      781 ccggcaactg gagcgggatc tgatggcata tggctgtgcc gttgagcagc tacccgcagg
      841 tgaactgaac agttgtggcc ggcgcgtgcg cttccaggcc ccc
//
