LOCUS       sequence-66411-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Procko E, Hedman R, Hamilton K, Seetharaman J, Fleishman SJ, Su M, 
            Aramini J, Kornhaber G, Hunt JF, Tong L, Montelione GT, Baker D
  TITLE     Computational design of a protein-based enzyme inhibitor.
  JOURNAL   J Mol Biol. 2013 Sep 23;425(18):3563-75. doi: 
            10.1016/j.jmb.2013.06.035.
  PUBMED    23827138
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2013.06"; journalName: "J Mol Biol"; date: 
            "2013-09-23- 23"; volume: "425"; issue: "18"; pages: "3563-75"
COMMENT     Sequence Label: pETCON-HtsptLB12v.3
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     101..124
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             456..467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             468..494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
       61 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
      121 cgtcaaggag aaaaaacccc ggatcgaatt ccctacttca tacattttca attaagatgc
      181 agttacttcg ctgtttttca atattttctg ttattgcttc agttttagca caggaactga
      241 caactatatg cgagcaaatc ccctcaccaa ctttagaatc gacgccgtac tctttgtcaa
      301 cgactactat tttggccaac gggaaggcaa tgcaaggagt ttttgaatat tacaaatcag
      361 taacgtttgt cagtaattgc ggttctcacc cctcaacaac tagcaaaggc agccccataa
      421 acacacagta tgtttttaag gacaatagct cgacgattga aggtagatac ccatacgacg
      481 ttccagacta cgctctgcag gctagtggtg gaggaggctc tggtggaggc ggtagcggag
      541 gcggagggtc ggctagccat atgggttccg aaatgagtga aatgtctgtt gtagattacg
      601 tcgttaagtc ttttaattat acagaggata tgagtcatga attgttattc tctgttaaaa
      661 agagatggtt cgtaaagcca ttcgaacact caaccagata tggtttgtta gcctacatgt
      721 tgttacctgg taactacata aagttcggtt tgtacgtttt aacgaaccaa gactatgcta
      781 gattcgaaat agcatgggta catgtcgata aagacggtaa aatcgaagaa agaaccgtat
      841 attccattga aacttactgg cacattttca ttgatatcga aaacgacttg aactgtccat
      901 acgttttggc aaagtttatt gaaatgagac ctgaattcga ta
//
