LOCUS       sequence-66496-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     Gourisankar Ghosh unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHis8-p100(1-899)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..161
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             168..185
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac catgaaacac caccaccacc accaccacca cggtggtctg gttccgcgtg
      181 gttcccatgg cggatccgaa ttcatggaga gttgctacaa cccaggtctg gatggtatta
      241 ttgaatatga tgatttcaaa ttgaactcct ccattgtgga acccaaggag ccagccccag
      301 aaacagctga tggcccctac ctggtgatcg tggaacagcc taagcagaga ggcttccgat
      361 ttcgatatgg ctgtgaaggc ccctcccatg gaggactgcc cggtgcctcc agtgagaagg
      421 gccgaaagac ctatcccact gtcaagatct gtaactacga gggaccagcc aagatcgagg
      481 tggacctggt aacacacagt gacccacctc gtgctcatgc ccacagtctg gtgggcaagc
      541 aatgctcgga gctggggatc tgcgccgttt ctgtggggcc caaggacatg actgcccaat
      601 ttaacaacct gggtgtcctg catgtgacta agaagaacat gatggggact atgatacaaa
      661 aacttcagag gcagcggctc cgctctaggc cccagggcct tacggaggcc gagcagcggg
      721 agctggagca agaggccaaa gaactgaaga aggtgatgga tctgagtata gtgcggctgc
      781 gcttctctgc cttccttaga gccagtgatg gctccttctc cctgcccctg aagccagtca
      841 tctcccagcc catccatgac agcaaatctc cgggggcatc aaacctgaag att
//
