LOCUS       sequence-66514-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt 
            CA
  TITLE     Characterization of 582 natural and synthetic terminators and 
            quantification of their design constraints.
  JOURNAL   Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
  PUBMED    23727987
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515."
COMMENT     Sequence Label: pGR-ECK120015170
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             76..87
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             94..807
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(124..152)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     747..767
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg
       61 ggctagctac tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact
      121 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      181 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      241 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa
      301 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc
      361 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      421 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      481 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      541 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      601 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      661 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      721 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      781 acacatggca tggatgaact atacaaataa taagaattca caattttcga aaaaacccgc
      841 ttcggcgggt ttttttatag ctaaaaacta gtagcggccg ctgc
//
