LOCUS       sequence-66758-e       10758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encode CRPV L1 and L2, Bicistronic, too large to self-package.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 10758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: pCRPVsheLL
FEATURES             Location/Qualifiers
     source          1..10758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3695..4283
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3748..3768)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4166..4177)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4320..4337)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4326..4550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4596..5024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4683..4702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4893..4914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5033..5053)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6982..7350
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     polyA_signal    7480..7601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7517..7536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7571..7590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7650..7666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7650..7666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7663..7685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7674..7690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7698..7704,7705..7722,7723..7728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7743..7764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7881..7898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8052..8637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8132..8151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8808..9599,9600..9668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9431..9450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9669..9773)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9848..9867)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10039..10418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10419..10622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10573..10593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10624..10642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10645..10663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10673..10697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccggtcgcc accatggccg tctggctcag cacccaaaac aaattctatc tcccccccca
       61 acccgttacc aaaatcccct caaccgacga atatgtcacc agaaccaatg tgttctacta
      121 cgccagttcc gatagattgt tgaccgtcgg ccacccctat tacgagatca gggacaaggg
      181 gacaatgttg gtccccaaag tcagccccaa tcagtatcgc gtgttccgca ttaagttgcc
      241 cgatcccaac aaattcgcct tcggcgataa acagctctac gaccccgaaa aagagagatt
      301 ggtttggtgt ttgcgcggca tcgaagttaa cagggggcaa cccttgggcg tgtccgtcac
      361 cggcaatccc attttcaaca agttcgacga cgtggaaaac ccaaccaaat actataacaa
      421 tcacgccgat caacaggatt atcgcaagtc aatggccttt gatccaaaac aagtccaact
      481 cctgatgttg ggctgcgtgc ccgcaaccgg cgagcattgg gcccaagcca aacaatgcgc
      541 cgaagacccc ccccagcaaa cagattgccc accaatcgag ttggtcaata ccgtcatcga
      601 ggacggcgat atgtgcgaga tcgggttcgg cgctatggat cacaagaccc tccaagcatc
      661 cctgagcgaa gtcccattgg aactggccca aagcatttca aagtaccccg attacttgaa
      721 gatgcagaag gaccaattcg gcgacagcat gttcttctac gcccgccgcg aacagatgta
      781 cgcccgccac ttcttttcac gcgccggcgg cgataaagag aacgtcaaat cacgcgcata
      841 catcaagagg acccaaatgc aaggcgaagc caacgcaaac atcgccaccg ataactattg
      901 tattaccccc tccgggagcc tcgtcagctc agacagccaa gtcttcaata gagcctactg
      961 gttgcaaaag gcccagggga tgaataacgg cgtctgttgg gataaccaga tcttcgtcac
     1021 cgtcgtggac aatacacgcg gcaccatcct gtccttggtt accaagagca aagacgccaa
     1081 ccaggaggat ccctggaaga attccacctt cagcagttac ttgcgccacg tcgaagaata
     1141 cgagctccag ttcgtcttgc aattgtgcaa agtcaaactg accccagaga atttgagcta
     1201 tttgcactca atgcatccca ccattatcga caactggcag ctcagcgtca gcgcccaacc
     1261 atccggcacc ttggaagatc aatatcgcta tctgcaaagc atcgccacaa agtgcccccc
     1321 acccgagcca ccaaaggaga ataccgatcc ctacaagaat tacaaattct gggaagtgga
     1381 cctgagcgaa aagttgagcg accaattgga ccaatacccc ttggggcgca aattcttgaa
     1441 ccagtccggg ctccagcgca tcggcaccaa gcgccccgcc cccgcccccg tctccatcgt
     1501 caagagcagc aagaggaaac gcaggacctg agctcgagga agcttaaaac agctctgggg
     1561 ttgtacccac cccagaggcc cacgtggcgg ctagtactcc ggtattgcgg tacccttgta
     1621 cgcctgtttt atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg
     1681 ggtacaaacc agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac
     1741 tgcttgcgtg gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt
     1801 accacctcgg aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc
     1861 tgatgagtct ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct
     1921 atggctaacg ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat
     1981 aagaatcctc cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc
     2041 agtgattggc ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt
     2101 gtttcctttt attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc
     2161 gaattggatt gcggccgctc tagcatggtc gccagaagca ggaagaggcg cgccgccccc
     2221 caagatatct accccacctg caagatcgcc gggaactgtc ccgccgacat tcaaaacaag
     2281 ttcgagaata agaccatcgc cgacaagatc ctccaatacg ggtcattggg cgtgttcttc
     2341 ggcggcttgg gcattagctc cgccggcggc agcggcggca ggttgggcta tacacccctg
     2401 agcggcggcg gcggcagagt catcgccgcc gcacccgtgc gccccccaat caccaccgag
     2461 agcgtggggc ccttggacat cgtccccgaa gtggccgacc ccggcggccc caccttggtc
     2521 agcttgcacg agctccccgc cgagaccccc tacgttagct caaccaacgt caccggcgac
     2581 ggggccgccg aaccattgcc cgccggccac ggcggctcac aaatcagcga tgtgaccagc
     2641 ggcaccagtg ggaccgtcag tcgcacccat atcaacaatc ccgtgtttga agcccccatg
     2701 acaggcgacc aagacgtcag cgacgtccac gtgttcgcac atagcgagtc ctccatcacc
     2761 atcaatcaga ccgagaatac cgggggcgaa ttgatcgaaa tggtcccatt gcgccatcca
     2821 ccccggtccg aaggcgactt tcgcgagacc agcttttcaa cctcaacccc caatcccgac
     2881 cggagcgccc tgaggagcat caatgtggcc tcacgccgct accaacaagt gcaggtcgag
     2941 aatcccgcat ttctcaatcg cccacgcgag ctcgtccaat ttgagaatac cttcgataac
     3001 cccgccttcg tcgacgacga acagttgtca ttgttgttcg agcaagactt ggataccgtg
     3061 gtcgccgccc ccgaccccgc ctttcaagac gtcgtcaggc tgtcccgccc aagctttacc
     3121 caaagtcgcg ccggccgcgt cagagtctca aggctcggcc gcaccttgac catgcagacc
     3181 aggtccggca aagcattcgg ccccgcaaag catttctatt acgaattgag ctcaatcgcc
     3241 gagggccccg aacccgatat cttgattcct gagagcgagc aagagaccag ctttaccgac
     3301 gcaaccagca aggataccca gcaagaggcc gaggtctacg ccgacggcag cacactggag
     3361 accgatacca gcgccgacga gaatctcacc ttggtcttct ccgatcgcgg gcgcggccaa
     3421 ggcagccacg tgcccatccc cgggaaaagt accatcggcg gccccatcaa catcggcgat
     3481 tcaaagtatt acaccctcaa tcccggcgag accacctcat tcgaggccga cgtgatcagc
     3541 cccgtcttta tcttcgaagg caatgccgac gggacctatt acttggaaga gcccttgagg
     3601 aaaaagcgca ggaagagcat tttcctgttg gccgacgggt ccgtcgccgt gtatgccgaa
     3661 tgagctcgag gctagcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3721 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3781 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3841 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3901 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3961 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4021 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4081 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4141 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4201 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4261 ctccctttgg gccgcctccc cgcatcgata ccgtcggccc gtttaaaccc gctgatcagc
     4321 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4381 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4441 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4501 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4561 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4801 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4921 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5041 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5101 cagaattcta tcaaatattt aaagaaaaaa aaattgtatc aactttctac aatctctttc
     5161 agaagacaga agcagaggga atacttccta aatcattcaa ctaggccagc attaccttaa
     5221 taccggaact agaaaatgac attacaagaa aagaaaacaa cagaccaata tctctcatga
     5281 acaaagatac aaacattttc aacaaaatat tagcaaaaag aatccaagaa tgtatcaaaa
     5341 aatatacacc acaaccaagt agaatttatt ccagatatgt aagggtggtt caacgtttga
     5401 aaatcaatta acgtaatttg tcccatcaac aggttaaaga agaaaatcac atggtcatat
     5461 tgatagacac agaaaaagca tttgacaaaa tttaacaccc attcatgatg caatctctca
     5521 gtaaactagg aatagaggaa aacttcctca gcttgaatgt accttcctct caattttgct
     5581 atgaacctga aactcctctt aaaaaataaa gtttttcatt taaaaagaaa acaaaaaaca
     5641 tggaggagcg ttgatgtatc tcattttaga ccaatcagct atggatagtt aggcgacagc
     5701 acagatagct gctgtacttc tgtttctggc aatgttccag actacattta aaaaattttt
     5761 aattatagac ttgtacttaa tgttcaagaa aaatatgaaa atggctttgc cgtgttaatg
     5821 ctactctttt ttaaaaaaaa ctaaagttca aactttattt atatttcatt agttttttag
     5881 ctactgttct ttttctgttc tgggatctca ttcagaatgc cacattacat ataattctca
     5941 tgtctccttg ggttcctctt agttttgaca gttcctcaga cttttcttat ttttgatgac
     6001 cttgacagtt ttgaggagta ctggttagat atagggtaat ggtttttaaa gtatatttgt
     6061 catgatttat actggggtaa gggtttgggg aggaagccca tggggtaaag tactgttctc
     6121 atcacatcat atcaaggtta tataccatca atattgccac agatgttact tagcctttta
     6181 atatttctct aatttagtgt atatgcaatg atagttctct gatttctgag attgagtttc
     6241 tcatgtgtaa tgattattta gagtttctct ttcatctgtt caaatttttg tctagtttta
     6301 ttttttactg atttgtaaga cttcttttta taatctgcat attacaattc tctttactgg
     6361 ggtgttgcaa atattttctg tcattctatg gcctgacttt tcttaatggt tttttaattt
     6421 taaaaataag tcttaatatt catgcaatct aattaacaat cttttctttg tggttaggac
     6481 tttgagtcat aagaaatttt tctctacact gaagtcatga tggcatgctt ctatattatt
     6541 ttctaaaaga tttaaagttt tgccttctcc atttagactt ataattcact ggaatttttt
     6601 tgtgtgtatg gtatgacata tgggttccct tttatttttt acatataaat atatttccct
     6661 gtttttctaa aaaagaaaaa gatcatcatt ttcccattgt aaaatgccat atttttttca
     6721 taggtcactt acatatatca atgggtctgt ttctgagctc tactctattt tatcagcctc
     6781 actgtctatc cccacacatc tcatgctttg ctctaaatct tgatatttag tggaacattc
     6841 tttcccattt tgttctacaa gaatattttt gttattgtct tttgggcttc tatatacatt
     6901 ttagaatgag gttggcaagt taacaaacag cttttttggg gtgaacatat tgactacaaa
     6961 tttatgtgga aagaaagtac caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7021 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7081 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7141 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7201 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7261 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7321 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7381 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7441 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7561 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7621 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7681 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7741 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7801 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7861 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7921 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7981 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8041 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8101 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8161 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8221 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8281 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8341 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8401 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8461 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8521 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8581 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9781 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9841 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9901 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9961 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    10021 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
    10081 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    10141 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    10201 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    10261 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    10321 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
    10381 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
    10441 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
    10501 tcaatgggag tttgttttgg aaccaaaatc aacgggactt tccaaaatgt cgtaacaact
    10561 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
    10621 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
    10681 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
    10741 accgggaccg atccagcc
//
