LOCUS       sequence-66806-e         214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for cloning and expressing your gene on 
            a bicistronic mRNA also encoding IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
  PUBMED    14603031
REFERENCE   2  (bases 1 to 214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jan 6. 101(1):16-22."
COMMENT     Sequence Label: pCAGIG
FEATURES             Location/Qualifiers
     source          1..214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..54
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     116..135
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
       61 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
      121 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga tatcgcggcc
      181 gctacgtaaa ttccgccccc cccccccctc tccc
//
