LOCUS       sequence-66809-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-terminal tal protein segments 
            increases mutation induction; Fok I DD (improved 
            version=pCS2TAL3-DDD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012-08"; volume: "8(8)"; pages: "e1002861. Epub 
            2012 Aug 16"
COMMENT     Sequence Label: pCS2TAL3-DD
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             125..145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             146..211
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     775..797
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     789..806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     790..806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     816..835
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        816..834
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(882..902)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(882..900)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(921..937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(921..937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ctgccaccat
      121 ggctccaaag aagaagcgta aggtagacta caaagaccat gacggtgatt ataaagatca
      181 tgacatcgat tacaaggatg acgatgacaa gggtaccgtg gatctacgca cgctcggcta
      241 cagtcagcag cagcaagaga agatcaaacc gaaggtgcgt tcgacagtgg cgcagcacca
      301 cgaggcactg gtgggccatg ggtttacaca cgcgcacatc gttgcgctca gccaacaccc
      361 ggcagcgtta gggaccgtcg ctgtcacgta tcagcacata atcacggcgt tgccagaggc
      421 gacacacgaa gacatcgttg gcgtcggcaa acagtggtcc ggcgcacgcg ccctggaggc
      481 cttgctcacg gatgcggggg agttgagagg tccgccgtta cagttggaca caggccaact
      541 tgtgaagatt gcaaaacgtg gcggcgtgac cgcaatggag gcagtgcatg catcgcgcaa
      601 tgcactgacg ggtgcccccc tggagacggg cgccgctaca gggcgcgtcc cattcgccat
      661 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
      721 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      781 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
      841 tgggtaccgg gccccccctc gaggtcctcc agcttttgtt ccctttagtg agggttaatt
      901 gcgcgcttgg cgtaatcatg gtcatagctg tttcctg
//
