LOCUS       sequence-66858-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b/EYFP-GgVcl 884-1066 (Vt)
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(625..876)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(667..688)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcctc gagctactga taccatgggg tctttctgac
       61 ccagcgcaga gtgaatccgg catctgttct tatcttaatg gatgctgctt cagcttctct
      121 cacagtttcc ttcacagact gcatgaggtt ctgggcgtta tgaaccaaca tctcagttgc
      181 ctgttctgat tcttcatcgc tgatgttagt cctgcccagc atggtagctt tcactgtgga
      241 aagaattttg agctgcgtgc tgatggttgg gattcgctca cagacctgta agaggtttgt
      301 tctaatgcgt ttatcagtac attgcttggc cacctctttg gccaatcgag tgacttcatc
      361 cgatgcctta gcaatatctt ttgcacactg gatgagggca cgcttgtttc cgctgcctcc
      421 tcgcaccagg cgtgacatct cagccatgag cagcgccatt cgtttagcag cagcaatgat
      481 gtcgttaccc ttgctagacc atttccgagc ctcgtcatgc aactgcctag cagccatcat
      541 catgggctga ttaatagctt ctcctgcttt ctgctctggg aactccatat gggcggcggc
      601 ggggcagggg gggccggaat tcgacttgta cagctcgtcc atgccgagag tgatcccggc
      661 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
      721 ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      781 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
      841 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
      901 gttgtagttg tactc
//
